Mokrini, F. Abbad Andaloussi, F., Waeyenberge, L., Viaene, N and Moens, M. (2014). β-1,4-endoglucanase gene suitable for the molecular quantification of the root-lesion nematode, Pratylenchus thornei, Nematology 16 (7), pp 789-796.
A real-time quantitative PCR assay was developed for the accurate detection and quantification of the root-lesion nematode, Pratylenchus thornei. A qPCR primer set, including two primers and a probe, was designed based on the sequence of the β-1,4-endoglucanase gene. The assay was optimised by using the primers with SYBR green I dye and setting the qPCR program to