Soil nematodes are major soil organisms known to reduce wheat production worldwide.However, they are a crucial part of soil biodiversity. Due to the limited data available regardingnematode biodiversity associated with cereal crops in Morocco, a survey was conducted in 2019across 80 wheat fields. Thirty-three nematode taxa were obtained with different trophic groups:11 bacterivores (i.e., Rhabditis and Cephalobus spp.), 11 herbivores (i.e., Pratylenchus and Heteroderaspp.), 6 fungivores (i.e., Ditylenchus and Aphelenchoides spp.), 4 omnivores (i.e., Dorylaimus andAporcelaimus spp.) and one predator (Mononchus spp.). For each locality, the diversity of nematodecommunities was assessed through multiple diversity indices. Three areas (Ait Melloul, Oulad Dahou,and Sidi Boushab) were more diversified and had pronounced nematode occurrence. Moreover, fewlocalities (e.g., Ait Amira and Lqliaa) were shown to be disturbed. Our study underlined that severalnematode taxa were significantly correlated with edaphic and climatic/geographic properties. Onthe other hand, the spatial and parsimony analyses revealed that plant feeders were most associatedwith bacterial feeders in disturbed soils. This work revealed that the soil nematodes associated withwheat crops are diverse and can serve as an important tool for soil biomonitoring at a large scale